Basic steps for installation and functioning of ReadPlate plugin in imageJ ?

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What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

What binder would be the best choice to modify glassy carbon electrode with a 2D Material like Graphene or MXene?

I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?

23 July 2024 5,776 2 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Which is the best approach for anomaly detection in scanned image data set?

Anomaly detection in scanned image data set

18 July 2024 3,578 3 View

What is the future scope of acoustic emission?

17 July 2024 1,510 1 View

Is the protecting group boc of the amino group stable at 37°C?

I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?

12 July 2024 3,745 2 View

Why can't I detect the plasmon resonance angle with water?

I am trying to measure the plasmon resonance angle of gold film and pure water using the Kretschmann configuration and a 633nm laser. Without flowing water over the gold, I can detect a clear...

10 July 2024 4,719 3 View

Does Nature Scientific Reports waive open access fee for industry authors?

I came across the Green Building and Sustainable Architecture collection under Nature Scientific Reports some weeks ago. https://www.nature.com/collections/gajghaebce The special issue/collection...

10 July 2024 5,533 1 View

Can I do Parallel Analysis for Principal Axis Factoring Method?

Recently I was suggested to do Parallel Analysis and compare with EV>1 to determine no of factors for my scale. However, the scale I have developed uses Principal Axis Factoring and not PCA. If...

30 June 2024 1,813 2 View

How can I better use imageJ to get good result on calculating surface area of a film accurately?

I have images of film formed on the bottom of a 50mL beaker, and I have been trying to figure out the surface area. I could easily use features on imageJ to do that, but I want to know which area...

23 July 2024 4,461 1 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

How to use ImageJ for fluorescence quantification of platelet adhesion and spreading?

I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...

11 July 2024 1,464 7 View

Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

ImageJ Concentric Circles?

Hi there! We are trying to use the Concentric Circles Plugin on ImageJ to determine signal intensity in neurosphere cryosections after immunocytochemistry/confocal analysis. We determined the...

12 June 2024 7,332 0 View

How to do image processing?

I want to analyze some images (nearly 1000) in a loop. I want to analyze HSV and RGB. I have masked those images in ImageJ, which is binary masked. I tried to explore them in R, but all the...

04 June 2024 5,098 1 View

How to quantify the fluorescence intensity of a confocal image with Fiji (ImageJ)?

I have taken fluorescence images of the control and treated sample(Immunofluorescence, tissue sample) at the same settings. So I need to measure the change in fluorescence intensity of the treated...

16 April 2024 2,093 1 View

What is the best method for quantifying lipid accumulation in C. elegans images using ImageJ?

We have a demarcation and analysis protocol in ImageJ for images of C. elegans stained with Oil Red O. However, it does not seem to be the best way and we were unable to find an easy-to-execute...

15 April 2024 2,031 1 View

What is the best way to normalize p-S6(Ser235/236) protein expression?

Hello everyone. I am in the middle of performing western blotting experiments measuring expression of p-S6(Ser235/236) protein in total cell lysate. Originally, I was quantifying my data using...

11 April 2024 4,457 1 View

How to count brown/brite adipocites on microscope?

How to count multilocular adipocytes on a slide with a white adipose tissue sample stained with H&E? I had done the count on the entire slide, but the articles put it in % of cells or...

10 April 2024 3,997 2 View