Hello,
I am working on mcyA gene in microcystis gene cluster.
I got stuck with motif concept. I understood that mcyA gene has 10 motifs and amino asid binding site or binding pocket between A4 and A5 motifs. There are positions numbered from A1 to A10 between A4 and A5 motifs. Substrate amino acid is bound with Asp235 and Lys517 first. That is ok.
Stachellaus et al. 1999 says that Asp235 is A4 position in binding pocket, but Kudo et al.2019 says that Asp235 is A1 position in binding pocket. Which one is correct? Am i missing something?
I am not biochemist, but i want to understand this part for understanding bioinformatic part better.
Thank you in advance
Gozde
Firstly, thank you very much.
Because I do not have full knowledge of the subject of amino acids as a chemists, I wanted to ask my question in here.
I will check the review and send e-mails to authors.
I sent an e-mail to author of the paper, Dr. Kudo.
He understood where i made a mistake. Asp235 is in A4 core motif (not A4 position) and can be called as A1 position.
Briefly, i confused with "position" and "core motif" terms while reading.
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