I introduced plant extracts during conjugation experiment and when viewed under electron microscopes, the cell wall and membrane became thicker and the lines became hardly visible. Any suggestions or publications I could refer to as to why this happened?
The plant extracts may have some tannins that have precipitated the cell wall proteins of the Bacteria.
Did the cell size also decrease? Maybe via the addition of the plant extracts you just increased your salt concentration which leads to a loss of water of the cells. Without knowing the components of your extracts it is difficult to guess.
Lalitraj Singh Thank you, I will read further on the subject.
Chinaza Godswill Awuchi thank you for your suggestion, I will check on the article.
Ainaa kindly read this
https://courses.lumenlearning.com/microbiology/chapter/unique-characteristics-of-prokaryotic-cells/
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