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Do 1-day protocols for miRNA in situ hybridization work for FFPE tissue sections?

The conventional protocols for miRNA hybridization for FFPE tissue sections stretch for 2-3 days, with prehybridization and an overnight hybridization steps. But some companies like thermo and...

21 June 2022 6,199 0 View

How to know regional groundwater flow direction??

I have water level data as well as elevation data. I just want to know the direction in which groundwater is flowing. I tried doing so using arc gis but I didn't help me much as it is giving me 8...

18 July 2021 4,622 8 View

What should be the standard glucose concentration to be used in high glucose studies, that match the physiological levels?

Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...

23 July 2019 2,088 4 View

How long we can store fungal spore suspension at 4 degree after counting spores with haemocytometer for next use ?

I am working on Plant Fungal Pathogens. I wish to know that after harvesting the fungal spores with 0.1 % Tween 20, how long we can store the spore suspension at 4C for next use ?

16 April 2019 8,650 12 View

Why conduction of anti parallel diode of MOSFET causes excessive heating in a bidirectional converter?

For a conventional BDC, the anti parallel diode of MOSFET gets heated up and draws high current after one or 2 minutes of conduction. I have tried with MOSFET IRF540 and TI MOSFET CSD19533KCS....

29 March 2019 7,106 0 View

What could be the possible reasons behind the cells dying in the culture plate in few wells while surviving in other, media conditions being same?

I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...

03 February 2019 1,772 3 View

At what stage of the cell cycle do cells arrest during subculturing process? Does the culture become synchronous after few passages?

During animal cell culture, confluent cells are trypsinised, centrifuged, resuspended and finally subcultured. I read somewhere that contact inhibition is used to make the cultures synchronous....

03 February 2019 561 3 View

Can anybody give me the composition of ALP buffer and how much substrate (PNPP) to be added in it?

I need to determine ALP activity in osteoblasts. I want to do it by colorimetric method but the reaction does not seem to work, even after incubating the plate at 37 for 1 hour I am not getting...

12 September 2017 8,491 2 View

How long and under what conditions can I store my derivatized GCMS samples?

I am using MSTFA, O-Methoxyamine in Dry pyridine to derivatize my samples. Usually I try to analyse the samples as soon as possible after derivatizing them. But I want to know whether I can store...

09 July 2017 4,183 5 View

Can anyone help me in doing GW calculations in vasp to obtain band structure?

Computational nanoscience

26 May 2017 9,684 5 View

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

A paper on a fossil lycopod?

Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...

07 August 2024 4,857 2 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View