Automated microscopes for fluorescence imaging ?

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Is Analysis of Similarity (ANOSIM) sensitive to differences in sample size?

Hi there. I want to compare 10 small forest landscapes on which different harvest designs were applied and see how much their attributes (structure, species, soil moisture...) differ. ANOSIM seems...

29 March 2020 5,826 4 View

Is there a way to download all molecules from a published research paper?

Hello, Is there a (preferably free) way to download all molecules (SMILES or SDF) from a published scientific article? Like searching pubchem or chembl or ZINC via DOI or so? I would like to...

05 January 2020 7,542 3 View

How to add a journal ?

Hello, is it possible to add a journal ? (Les Cahiers de Neuropsychologie Clinique) Some of my references can't be complete. Thank you.

08 October 2019 8,622 3 View

What would be an appropriate method to compute the watershed of a lake?

Hi there, I'm wondering what would be the best approach to create the watershed of a lake. I see two solutions here: - Creating the watersheds of all tributaries of the lake and aggregate them,...

02 March 2019 2,527 3 View

Is surface water supply a hydrologic service, a forest service, or a watershed service?

There is no doubt that the access to surface freshwater is an ecosystem service. However, I feel that the many publications on ecosystem services and disservices with respect to the water supply...

07 January 2019 4,166 13 View

What are your tips for obtaining maximum resolution on an agarose gel?

I want to resolve very small deletions on agarose gel. My fragments are ~ 200 bp. What are your tips to get the best possible resolution? Here is what I have already gathered around: - 4%...

18 November 2018 5,740 6 View

What is your favourite long-range PCR enzyme?

I want to amplify a long region of around 20 kb from human genomic DNA. I understand this is around the maximum of what is feasible, but looks possible, at least according to manufacturers'...

17 June 2018 4,218 6 View

I am looking for post-doctoral position that may begin in the fall. Can you help?

I defended my dissertation for the Payson Program for Global Development at Tulane University in May and I am currently working on the required edits. The title of my dissertation is "A...

05 June 2018 3,032 0 View

How to install Neo (Python package for working with electrophysiology) for MAC?

I am wondering if it is possible to install Neo (Python package for working with electrophysiology) http://neo.readthedocs.io/en/0.5.0/ on a Mac. If yes, how to do it?

21 February 2018 2,857 1 View

Anyone using Brookhaven BIC90 for zeta potential measurements. What is the polymer of the Organic Solvent Electrode?

I need to measure zeta potential of dispersions in organic solvent NMP DMF. I can't seem to find the material of the electrode, what polymer and it is compatible with the solvents.

02 January 2018 8,876 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Jan-Niklas May

We are using a Vectra 3 from (former) PerkinElmer, in between the whole department was bought by Akoya Biosciences. Has nice acquisition and analysis software included, however, I would suggest to buy the Polaris (successor of Vectra). Have no information about their other systems. No contact with the Akoya staff so far, but apparently, they took over the support team from PerkinElmer which was great. Multiplex stainings are cumbersome to establish but can be quite powerful.

Francois Piumi

Thanks for your fast and detailled answers!

Jason A Kilgore

I recommend the EVOS M7000 with the additional Onstage Incubator system. But I do tech support for the company that sells it, so I guess I can't claim neutrality (though I'm no sales guy). If you're interested, I'm happy to direct you to info.