Generally, for pulses we are applying little quantitiy of nitrogeneous fertilizer say, 25 Kg ha at the time of sowing. Considering after 25-30 days require for their better nodulation and formation therafter they able to utilize their nitrogen requirment from atmosphere via nodule.
As most of the legumes having 60 to 67 days duration except Pigeon pea its duration more than 4 months. Crop like Green gram, B gram, H gram if you obsrved its phenohases are interveling within short period.
Nodule biology is very important that type of nodule, content of leghemoglobin etc.
From my experience and reading legume nodules will be apparent at 20 to 30 days after planting. They will increase in their metabolism during the next 20 to 30 days. At that time when a crop like soybean is in full reproductive stage from 65 to 90 days the nodules will undergo senescence. As you commented for longer duration crops the periods with be extended and in longer duration crops the fixation in total is more. The fixation from the bacteria is related to mass and activity of the nodules.
The nodulation can be enhanced by rihizobium treatment. However, the most important thing is duration of crop. As duration in case of Red gram affect the nitrogen fixation capacity.
The short lived crop plant fix less nitrogen.
And the stage is concern the Branching is the stage in legume where maximum possibility of nitrogen fixation.
I agree with your opinion Dr Paul and Rajemahadik sir. Possibly flowering to pod development stage may be higher nitrogen fixation I.e. particularly during intermediate stage of crop. I think, the healthy, big size and higher modulations can be observed during intermediate stage leads to higher nitrogen fixation also due to high microbial interactions and peak period of nutrients demand.
Higher nitrogen fixation is up to their limit as we know most of the pulse fix atm. Nitrogen 40 to 80 kg per ha. So its depends upon duration, tupe of crop, species etc.
I agree with your opinion Rajemahadik sir. Possibly species and cropwise may be varied. We can't change cycle, duration, etc. but overall it may be happening when there is need for crops.
From our experience with soybean, N-fixation peak around 8 weeks and then decline to about 14 weeks, but it will depend on the maturity class and how fast they develop. We use a group V maturity class.
From branching to flowering stage, nitrogen fixation in legumes is highest. In most of the legumes, branching starts from the 3rd week after planting. At pod formation and pod-filling stage, plants preclude energy diversion to nodules, leading to their degeneration in most legumes. In annual legumes (beans, peanuts, soybeans), the duration of nitrogen fixation is shorter than that of perennial legumes (pigeonpea, alfalfa, clover) species. Nodules of annual species are relatively short lived. The amount of nitrogen fixation varies with legume species, Rhizobium treatment, the amount of available P in the soil, and the like. For ready reference, you may hit the link given below: