I intend to use sucrose gradient, but don't know what speed to use and how long. Any suggestions would be appreciated
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hi everyone, I run PCR to checked presence of specific gene in Clostridium perfringens but I received 2 bands, one band showed product size expected and another one showed higher product size. I...
31 May 2024 645 1 View
I want to do PCR amplification with my full-length gene and the addition of P2A fragment at the end of my gene. However after doing PCR, I run gel electrophoresis for analysis. But it doesn't...
03 May 2024 9,432 5 View
I am a Master's student now and I am currently working on a project related to mRNA loading lipid nanoparticles and I want to check the translation ability of mRNA on HEK293T cell lines (I will...
23 April 2024 5,321 1 View
In zinc air battery, the capacity is calculated according to the mass consumption of zinc metal. In experiment, how to determine the mass consumption of zinc metal?
15 April 2024 1,667 2 View
"The BERQ can be used for free for research purposes. Please mail the authors to request reprints of the questionnaire." pls help me, i dont understand what is REPRINT while it FOR FREE for...
26 March 2024 6,904 0 View
Hello everybody, I'm currently performing Western blot and I am a beginners for Western blot, so I want to ask that Can I replace blotting paper by any other papers?
14 March 2024 1,877 3 View
Hi everyone, Recently, I bought a new cell line named Tenocytes from a company. https://www.angioproteomie.com/ccp6601-human-tendon-cells-28tenocytes29-cap-0041.htm I followed the...
07 March 2024 6,149 3 View
As the title says, I am wondering about how to improve the model's speed by doing some hardwares changing (mainly processor, socket 1700/AM5 backward) aside model tweaking. Any help would be...
26 February 2024 1,584 0 View
Hi Everybody. I'm senior in a University in Vietnam. This is fisrt time I do SDS-PAGE, so I want to ask about the correction of my loading buffer recipe for SDS-PAGE as a following file. In...
26 February 2024 7,565 3 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
In cases where the rotational of the magnetic field H is zero, we can define this field as the gradient of a scalar function defined as the magnetic scalar potential (similar to the electric...
21 July 2024 9,633 4 View
Where is the stream gradient usually greatest and relationship between the slope of the stream channel and the velocity of the stream?
13 July 2024 5,825 0 View
How do gradient and discharge change in a downstream direction and why are glacial valleys shaped differently from river valleys?
13 July 2024 9,228 3 View
What happens to the stream's discharge if the gradient of a stream increases and relationship between the gradient of a stream and the rate of water flow?
13 July 2024 5,085 2 View
How does that stream's gradient change downstream as it enter the alluvial fan and difference between an alluvial plain and a delta plain?
12 July 2024 7,337 0 View
How does stream gradient steepness change moving downstream and gradient of a stream change along its longitudinal profile?
12 July 2024 4,113 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...
24 June 2024 6,079 4 View
Hi, I have recently ran a duplex ddPCR EvaGreen assay using different primer concentration for target (150nM) and reference (50nM) primer. The assay was ran on a XY sample for SRY copy number...
22 June 2024 9,200 3 View