Beyond Beall's list, how to identify phony versus legit journals?

Make no mistake that journals indexed in reputable databases such as Scopus can be considered legit by default. Your contribution sure whets your appetite for learning which venue should...

27 October 2020 246 5 View

How can I analyze the TGA graph to get the weight percentage of organic matter?

Dear everyone, This is the first time I analyze TGA graph of my materials including protein and cooper phosphate crystals. And I would like to measure the weight percentage/ loading percentage of...

09 September 2020 1,062 3 View

How to obtain clear Ru 3d5/2 peaks?

I am trying to obtain Ru 3d XPS spectra of samples with RuO2 content of 0.1 ~ 0.3%. But due to the overlapping of the C 1s region and maybe the low content as well, the Ru 3d5/2 peak was almost...

22 July 2020 7,789 4 View

Any recommendation for PhD research topic in consumer culture?

Hello everyone, I am stuck with my PhD proposal ideas. I want to focus on globalization and cosmopolitan consumers. I would be very appreciate if you can provide me some key papers and suggestion...

24 March 2020 8,227 3 View

Configuration of HPLC system ?

Dear all, Does there anyone know how to configure the HPLC system? Our Waters HPLC instruments (Waters pump 1525 and PDA 2998) don't occur in the list of Waters DHCP server configuration even we...

07 January 2020 4,636 2 View

Could you suggest ways to improve the strength of a generated monoclonal antibody? Can we clone mAbs from infected host animals such as cows,.. ?

Dear everyone, We generated mAbs via hybridoma systems. The mAbs show high antigen binding affinity and specificty. However I still wonder what is the best way improve the strength of the mAb? To...

26 November 2019 5,914 3 View

How many interviews (qualitative data) are needed to support quantitative data?

I'm doing my MA dissertation on writing strategies usage. I got 69 questionnaire response. I was wondering how many interviews I should do to support the quantitative data? Thank you

14 August 2019 8,937 7 View

Valves for elemental mercury related experiments?

Hi, Does anyone know which type/brand of valve is usable in case of gas flow containing elemental mercury? Extra information: Our lab is working on a project related to mercury adsorption and...

17 June 2019 3,758 3 View

Command line for results in autodock4?

Dear professors, Could you please tell me the command line to transfer dlg results file into pdbqt files in COMMAND PROMT? Im very hopeful for your advises. Kind regards.

18 May 2019 4,205 2 View

Add charges to Zn in protein?

I am now using Autodock Tools and Autodock 4 to figure out the interaction of ligand-protein. However, there is a problem I have to face now is that the Zn in the protein has 0 charge instead of...

15 May 2019 4,171 5 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

PCR primers bind to specific DNA at specific temperatures but not other temperatures?

I ran PCR in order to analyze a P-element excision on a mutant drosophila line. My results were not conclusive, however that is beside the point. I ran a temperature gradient in order to optimize...

25 January 2021 9,164 11 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

How to check "no blast hit" sequences of NGS data output?

My environmental sample was analyzed for fungi by both DGGE (18S) and NGS (ITS2), resulted in some different names (in the top 5 of strong bands in DGGE, or most abundant sequences in NGS) between...

21 January 2021 5,310 3 View

Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View