How nessesary is working point zero charges for any adesorbent?

How nessesary is working point zero charges for any adesorbent? I want to know how suitable the surface of the material is good for adsorption. I got SEM and FTIR results and thought those are...

30 January 2021 5,120 2 View

What are the parameters for sustainable fibres for concrete?

28 June 2020 1,987 1 View

On what issues the performance of Neural Network mainly depends?

On what issues the performance of Neural Network mainly depends?

20 January 2019 4,434 8 View

Does the pre-induction OD600 affect expression of stable proteins from bacteria?

We often induce protein expression when OD600 of E. coli culture reaches 0.4-0.8. What is/are the effect(s) of the pre-induction OD600 on expression level and stability of target proteins from...

19 March 2018 767 2 View

I am able to detect DNA bands on agarose gel from negative controls of PCR products? Did any one comeacross the same problem?

Recently, I have performed PCR reactions to amplify different gene fragments for cloning. I usually included negative controls in my experiments. The negative controls contain all the reaction...

29 August 2017 9,201 9 View

Why do HIV vaccine candidates developed so far fail to show good protective efficacy during preclinical and/or clinical trials?

It has been more than three decades since HIV/AIDS has already spread throughout all the five continents (North America, South America, Europe, Africa, Asia and Australia). A lot has been done to...

17 July 2017 709 6 View

Best software for amplicon seq data analysis for malaria parasite.

14 July 2017 2,025 1 View

Data analysis using qiime for malaria parasite clones

14 July 2017 4,585 2 View

Is it possible to engineer E. coli strains that do not produce endotoxins?

E. coli strains are good working horses for expressions of recombinant proteins. They have got huge potential for production of low-cost subunit vaccine candidates against various target diseases....

18 June 2017 9,754 11 View

Studies that evaluated the effectiveness of diarrhea intervention used longitudinal prevalence and incidence.

Any explanations for calculating relative risk (RR) for incidence and odds ratio (OR) for the longitudinal prevalence of diarrhea in randomized controlled trials to evaluate the effectiveness of...

18 December 2012 9,256 2 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

What will happen when we assume an upper bound of OFV lower than the global minimum OFV ?

if we assume 20 is the upper bound of an objective function (OF) based on permutation a square matrix (nxn) , The minimum value of the OF=22, then we run a random operator to compute the OF by...

14 February 2021 6,056 5 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View