Why Cutting force coefficients or the edge coefficients will be negative in few cases of machining operation?

In mechanistic modeling of cutting forces in turning, identification of cutting force coefficients and edge coefficients through experiments are very much essential. these coefficients can be...

05 October 2019 3,864 3 View

What can control gene expression after irradiation (besides DNA methylation)?

I'm studying a gene whose expression is regulated by DNA methylation. Cancer cell lines that express this gene show strong hypomethylation, while cells lines that don't express it show...

19 March 2019 7,845 2 View

How to solve scattering problem in sidewall of any pattern devices by using maxwell formulation without simulations through FDTD and COMSOL?

Actually i am looking some theoretical model who can solve the semiconductor scattering issues.

22 August 2018 3,062 2 View

Is it possible to get yield more than 100% in a chemical reaction....?

Is it possible to get yield of a chemical product more than 100%...? If so, how one can defend this situation? I am working on Condensation reactions using organic solvent free synthesis. For some...

22 June 2018 2,453 21 View

Can I transfect the only 147nt ssOdna 5'-3' (only sense strand)as repair template for Knockin experiment?

27 August 2017 3,856 2 View

Hi - does anyone have any advice about retrieving PBMCs from their suspension in RNAlater?

The PBMCs were suspended in RNALter and then stored at -80oc. Now I want to retrieve the PBMCs and then extract the RNA (inc the microRNAs) - does anyone have advice about centrifuge speeds/...

19 June 2017 6,038 3 View

Molecular weight changes with GPI Anchors

I'm studying a GPI-anchored protein and all of the antibodies against this protein are ~5-7kD higher than the expected molecular weight (expected is ~18kD and most antibodies give ~25kD). I'm...

21 May 2017 1,323 5 View

Does anyone help me out to clone the Oligo sequence in Lenti CRISPRv2 backbone plasmid ?

18 May 2017 6,919 1 View

In ABAQUS: How to apply pressure loads at different locations?

In ABAQUS: How to apply pressure loads at different locations?

07 May 2017 1,676 4 View

Is there any way to in vitro methods for checking to anticancer properties of plant extracted substance without using animal cell line or MTT assay?

I am working on the phara quantification of plant substance, facing some experimental aspect , alike no availability of animal cell culture setup. So, anticancer assay aspect completely non in...

20 January 2017 8,296 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

What is the best antimicrobial agent among metal oxides?

I am looking for an antimicrobial metal oxide agent for a polymeric implant and I am wondering which one is the best?

21 January 2021 7,068 5 View

Does anyone know a good antibody anti c-Fos working on fish ?

I know this has been asked before, I repropose the question hoping to get an up to date fedeback. I have already tested the E-8 and 6-2H-2F from Santa Cruz with really poor results. Unfortunately...

19 January 2021 6,270 2 View

How should I prepare my tissue sample for biodistribution study?

I want to evaluate the biodistribution of an elastin-based nanoparticle drug in tumor, heart, liver, and kidney for the first time. Can anyone give me any idea about how to prepare my tissue...

04 January 2021 3,661 3 View

Is it indispensable to receive a vaccine by the COVID-19 recovered person?

Is it indispensable to receive a vaccine by the COVID-19 recovered person?

22 December 2020 5,883 5 View

What would cause different shape DMA loss modulus curve in different batches of PC+ABS?

I have some DMA curves of PC+ABS from different batches. The PC loss modulus peak is fairly consistent but one batch has a peak value 10degC lower than the rest. Another peaks at the normal value,...

06 December 2020 809 2 View

Is there any possibility to buy specific drug resistant human and mouse tissue array from company or other research organisations?

I need some specific cancer tissue samples' slides for IHC which is resistant and sensitive to specific cancer drugs, especially for prostate, lung, and breast cancer. Is it possible to buy or...

27 November 2020 731 2 View