Why is ccdb toxin not killing susceptible strains of E.coli?

Hi hive, We are trying to use ccdb as a counter selection marker. We are using the cassette from this paper, which should be highly expressed: A new method to customize protein expression vectors...

22 June 2020 5,084 5 View

Appropriate statistical test for within-group comparisons of questionnaires?

Dear colleagues, I am trying to determine the appropriate statistical test for my problem. I have read several discussions on the topic here, however was unable to extract an answer, and so I...

03 May 2020 6,281 10 View

How to interpret transition states whose Gibbs-energies are higher than the Gibbs-energies of their corresponding ground states?

I am performing DFT-calculations (gamess-us) on a reaction: ground state 1 -> transitition state -> ground state 2. (in short: gs1 -> ts -> gs2.) ts is only 0.3 kcal/mol higher in...

16 February 2020 2,261 1 View

Open datasets for Machine Learning in geotechnics / engineering geology?

Machine learning has proven to be very successfull in many disciplines and the general development is surely furthered by openly accesible algorithms and datasets for the whole community to work...

23 October 2019 6,941 19 View

Is it possible to produce a device that can project screen out of the mind in physical - how is the theory of that?

Can there exist societysystem including projections of pictures and video frames out of thought into the physical reality? Is this possible for nature of human beeings or has this to be technical...

30 May 2019 2,426 5 View

Secret of neural stem cells?

Hi! I can not find a list of growth factors that secrete neuronal stem cells. Usually everyone writes about neurotrophins. But I think there should be other factors ( fgf, egf, igf, vegf)?

12 November 2018 9,084 2 View

How to conduct power and sample size calculations for the probit model with binary covariate(s)?

I would like to calculate the optimal sample size (based on power calculations) for the probit model and a binary covariate.I have run a pilot study for a randomized control trial. I am interested...

03 September 2017 5,895 3 View

Hydrophilicity of PDMS due to treatment with Piranha and KOH. Treatment time?

Apart from plasma activation, PDMS can be made hydrophilic by treating it with Piranha solution (and maybe KOH in an elaborate approach). I found two papers reporting this effect. Interestingly,...

18 April 2017 1,857 4 View

Could anybody provide me with a table for the redshift and distance of at least 30 galaxies within 1 billion light-years?

Additionally details of measurement for the distance would be appreciated, but is not mandatory.

01 November 2012 2,944 2 View

I would like to hint to my projects related?

Please have a look on my publications Georg Oesterdiekhoff For example Child and ancient man American Journal of Psychology 2016

01 January 1970 8,102 5 View

How to get the heading of real-time credit card dataset ?

I am working with the Credit card dataset. I am aware of the fact that the Banks and financial institutions do not share the dataset with third-party companies or researchers. I just need to know...

18 February 2021 9,186 3 View

Can we help fellow mathematicians get free ?

https://terrytao.wordpress.com/2021/02/08/a-statement-from-mathematics-department-faculty-at-stanford-and-mit/ Terry Tao blogged about this unfortunate event. Kindly share among Mathematical...

08 February 2021 1,055 3 View

How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

Can I use video interview excerpts from others in public domain (YouTube, Vimeo, etc) for my empirical analysis?

Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...

31 January 2021 3,438 3 View

Is aligning mitochondrial and nuclear sequences inconsequential?

Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...

19 January 2021 5,895 2 View

How to estimate the standard deviation of the carbom emission of a company producing many different products?

I am aware of the portfolio theory in general but want to know of a journal or author especialized in this issue.

14 January 2021 7,566 2 View

Which method to choose for analysing semi-structrured interviews?

Hi, I did a literature review on innovation diffusion theories (Rogers), and I attempted to test the practicality of these theories when introducing novel medical devices. I did semi-structured...

05 January 2021 276 8 View

In your opinion, what could motivate people to wear masks and do social distancing to stop covid-19?

28 December 2020 5,149 56 View

HRM using Stratagene?

27 December 2020 868 4 View