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More Ruggero Romagnoli's questions See All

P21 molecular weight?

I'm working on senescence, and I'm trying to observe senescence markers. I have performed western blots for p21 but it seems that the bands are at a much higher molecular weight than expected. The...

22 March 2024 9,280 2 View

Can you culture HUVECs with DMEM ?

Hi, I am culturing HUVECs using the Endothelial Growth Media from PromoCell, however is pretty expensive. Does anyone know any alternative? is it possible to use DMEM for culturing HUVECs?

12 September 2023 9,494 1 View

Which are the best cytokines to use as markers for the senescence associated secretome phenotype (SASP)?

Hello, I'm working on senescence and I'm inducing it using TNF alpha. I would like to assess the induction of the phenotype looking for the cytokines typical of the secretome. Which cytokines do...

19 September 2022 2,258 3 View

How to correctly employ an aluminium sacrificial anode in a constant potential electrosynthesis?

Hello everyone. We have recently started a research on the electrochemical fixation of carbon dioxide on organic substrates (i.e. electro-carboxylation) and we are currently investigating the...

11 March 2021 2,913 0 View

How to deal with Single and Multiple Item latent variables in Structural Equation Modeling?

Hello all, I have a theoretical question. I am trying to run SEM, but one of my latent variables have 24 items as an indicator, and whenever I use it, the fit of the model goes down. So I thought...

29 March 2020 7,020 27 View

To what extent do you need a detailed and / or a priori literature review for an inductive qualitative study?

I am new to transparently inductive research, coming from a much more quantitative background. Really trying to do it as thoroughly as possible as I can see it's often done badly! But there's a...

05 December 2018 3,135 9 View

Dear all, I'm looking for a crocidolite sample (around 1-200 gr) possibly from a known location and clearly fibrous, can anyone help?

In order to complete our experiments we need around 1-200 grams of crocidolite with clear fibrous habit. Thanks

10 December 2017 8,053 0 View

I have to do the spore germination assay on dermatophytes. Can someone help me?

I need some exhaustive indication on the spore germination assay that I have to do on dermatophytes. The complete method would be nice. Thanks

14 January 2015 7,756 1 View

Where can I find information about how frequently multinational companies relocate their production facilities?

I would like to know if there are statistics or researchers showing how frequently multinational companies relocate their production facilities around the globe. I am not interested in new...

07 December 2014 4,912 2 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

London dispersion in CCl4 vs the dipole-dipoles force strength in CHCl3?

How do you tie-break and rank these two in their strength?

03 August 2024 4,471 1 View

Training for new staff?

I am looking for some training for new staff that will be starting in a self contained classroom with students with ASD. Most new staff have little to no experience working with students with ASD....

03 August 2024 6,717 3 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another?

Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another? Which leadership training methodology would be most successful with your...

14 July 2024 173 4 View

What is the mathematical expressionof theoretical yield strength calculated considering solution strengthening in stainless steel 316L?

In the expression for solid solution strengthening model that has been widely used in the literature use a linear form consisting of strength coefficient, and concentration of elements in wt.%....

11 July 2024 4,476 3 View

Short communication journals for mini review submission?

Is it possible to submit a mini review in short communication. Please suggest some journals accepting mini review as a short communication.

08 July 2024 8,553 1 View

Is there any research paper on impact of knowledge sharing, training and development on employees retention??

I want to make thesis on this topic is it right??

06 July 2024 7,101 5 View

Can someone explain the pros and cons of mean squares vs. Baker's ratio for pinpointing gene action in line x tester designs?

In plant breeding research, line x tester mating designs are often used to assess gene action for various traits. Some studies suggest that the simultaneous significant mean squares for lines,...

30 June 2024 4,877 2 View