Under 400x magnification using a child’s light microscope (TK2 Scope), I observed a wet yogurt slide With blue stain.
These circular and various particles floating. Are these live bacteria OR milk fat globules? I am trying to verify if Oikos Triple Zero Greek Yogurt (supposed to be fat free) contains live probiotics. Yogurt was mixed with water and a drop was placed onto a microscope slide with cover slip.
But hard to distinguish with the limited magnification of the TK2 Scope. These circular floating particles also seemed perfectly round/sphere. Which led me to believe they are milk fat globules? But according to the label, the Oikos Triple Zero Greek yogurt is fat free.. which is supposed to remove the fat globules right? Trying to find rod-shape bacteria was unsuccessful which makes it hard to verify if there are live bacteria or not in these yogurt. But In the final video (4556.mov)I think I found some Streptococcus thermophilus. The shapes are spherical and appeared in pairs and some even connected to form a chain. Please review all of the videos.
Could you take a look and let me know your thoughts? If you find/verify bacteria, could you take a screen shot and labeled it?
It's very difficult to observe on your videos. Try to make a starter cultures of your samples to validate your hypothesis
Here Streptococcus Thermophilus Gram Stain :
https://www.researchgate.net/publication/332043319/figure/fig1/AS:741446533533702@1553786037813/Streptococcus-thermophilus.ppm
Is the video blurry? Or is it because the magnification is limited therefore difficult to see bacteria shape/structure?
Try to validate your hypotheses by microbiology or molecular biology. If you have bacteria actively growing, they will be more easily identifiable by microscopy.
Thanks but I am using a kid’s microscope and observing at home. I don’t have access to molecular biology equipment at home to verify. Therefore, I am limited to just observation at 400 magnification for live bacteria in this retail bought yogurt.
It looks like bacteria, but it seems a bit difficult to make a definitive diagnosis this way. It would be healthier to look at it at the gene level. Thanks, Good luck.
Christopher Xiong ......Christopher, I read the responses and decided to ignore your video examples. If you want to look at yogurt and its probiotics you'll need to obtain or borrow much better tools. - The Univ of North Carolina has hundreds of labs with microscopes you can use !!!! -- In addition, please do a search in Youtube (see #1 below) for “Yogurt Probiotics Under Microscope”. There’s many other examples with most identify the probiotic strains. Depending on what you’re trying to learn I suggest broadening the search to the internet using “Images” and “All”
1)__ Results of a Youtube search for Yogurt Probiotics Under Microscope
https://www.youtube.com/results?search_query=Yogurt+Probiotics+Under+Microscope+
2)__ Probiotics under microscope
https://www.youtube.com/watch?v=J1QgSG1gONM
3)__ Probiotic Capsules Under Microscope (Do Dry Probiotics Work?)
https://www.youtube.com/watch?v=bz4n_GkdU7s
4)__ Chobani Yogurt Under Microscope (Probiotics)
https://www.youtube.com/watch?v=1aTpVEfcnOI
5)__ Microscopic-Analysis-Probiotic-Foods
https://www.ninolab.se/wp-content/uploads/ProductDoc/LLI/Mikroskop/EN_wp_Microscopic-Analysis-Probiotic-Foods.pdf
Christopher Xiong Christopher, Please continue looking at everything you encounter under your TK2 microscope. I'm adding this to say what you'll see on the macro and micro scale matters more the the quality of tools. The best thing I saw was how similar the make up of parts of the natural world are.
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