I wanted to know about techniques to estimate lipid of nematodes even with small number of nematodes (100 to 200 individuals) other than staining. Where can I get the protocol for that if possible.
Jarlei Fiamoncini
I would try to extract the lipids using a traditional method (Folch, for example) and then measuring triacylglycerols with an enzymatic kit.
I am not sure of the amount of sample that you have available (in mg) but this method is sufficient to quantitate triacylglycerols in as less as 20 mg of liver.
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