Membrane protein missing from SDS-PAGE gel?

I am trying to run an SDS-PAGE after his-tag purification of a sample. The protein of interest is an oligomerising membrane protein with an YFP tag attached. When the fractions of the sample from...

07 September 2020 3,277 6 View

Tool to calculate lag between BOLD time series?

Dear community I would like to ask if you are aware of any tool (Matlab/toolbox, etc.) that calculates the "lag between BOLD time series", as described in the following publications:...

01 November 2018 2,428 2 View

Is it necessary to make students prepare class reports as well as insect collections?

Dear All, I need the experience, data and opinion of zoologists/entomologist teaching zoology/entomology for BSc or MSc courses. It is necessary to make students prepare reports on the...

18 May 2017 7,405 15 View

T47D and A2780 growth problem.Why?

We work for many years with different gynecological cancer cell lines with the usual conditions. Two of these are T47D (breast) and A2780 (ovarium). For a month, this two cell lines are growing...

26 March 2017 5,358 7 View

Is there any side effects of eating rice or grains with read meat?

Red meat is harder to digest than for example poultry. If you eat a high carb meal with red meat the higher ptyalin production can increase the pH in the stomach resulting in bad digestion. Is...

03 March 2017 2,602 10 View

In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,734 2 View

Does anyone know about a Gene Expression Library (RNA-Seq), containing gene expression values across different cell-types and cell lines?

The ones I could find so far are highly biased towards cancer, and I needed a representative library. Does anyone know about such resource? Thanks

09 January 2017 122 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

No title

01 March 2016 5,806 6 View

In MTT assay a cancer cell line growth activity (untreated control cells) is decreased in repeated measurements. Why?

We usually use MTT assay with more cervix carcinoma cell lines to detect antiproliferative effect of drugs. At the end of these measurements we get absorbance values according to produced formazan...

13 October 2015 9,429 6 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

No title

22 February 2021 9,932 11 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View