Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

How to correctly indicate a zinc finger in a protein for molecular dynamics?

I am preparing a protein that has a Zn finger for molecular dynamics. To indicate the finger of Zn a patch is placed in which the deprotonation of the cysteines is necessary so that the Zn ion can...

05 March 2020 1,425 3 View

No title

28 February 2020 1,633 2 View

How do I calculate solar absorbance and thermal emittance of absorber coating from reflectance spectrum using standard AM 1.5 and blackbody radiation?

I have a reflectance data of my solar absorber coating from UV-Vis, I want to calculate the solar absorbance of my coating using standard reference spectrum AM 1.5 and thermal emittance of thesame...

15 November 2019 225 3 View

How can Iimprove the resolution on a GC?

When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just...

22 August 2019 4,050 6 View

On a EVO LS10 SEM, I can see images on spot 0 of the aperture, however on spot 1 and 2, I cant see any images. what could be responsible for this?

I an using the EVO LS10 SEM from Zeiss, I can see images on spot 0 of the aperture, however when the aperture is switched to spot 1 or 2, I am unable to see any image. Please what could be...

05 February 2019 3,902 1 View

Where to get yeast W303-K6001 strain?

15 September 2018 3,735 2 View

My solvent-casted PVDF film was opaque and somehow shrunk during vacuum drying. What should I do?

I'm trying to make smooth PVDF film using solvent casting dried under vacuum from 20 mL of 15% PVDF in DMF solution. The vacuum dryer temperature was at 55 deg C. After some time, this is what I...

29 January 2018 803 4 View

How can I get rid of the polysaccharides on orchids tubers to isolate DNA?

I try to isolate DNA from orchids tubers, the problem is they become kind of gelatinous because the polysaccharides.... so I want to get rid of them. Thank you!

18 September 2017 9,815 3 View

Shuold the "Reads" be included or taken into consideration for calculating the researcher's score in RG?

Any RG's member

02 March 2017 6,338 8 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View