Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Do Fire damages Concrete Structures Below soil?

Hi All, I am working on the rehabilitation of a fire-damaged structure. Should I consider the Column below Soil structurally deficient. visually it doesn't look like fire affected the column...

12 January 2021 5,401 3 View

Are there any Uni-Dimensional item Scales available for Social Media Marketing? kindly recommend me articles or item scales for Uni-dimensional SSM ?

I couldn't find Social media marketing uni dimensional item scales. Kindly recommend or share with me articles having only uni dimensional measurement scales of Social media marketing to check its...

09 November 2020 8,793 3 View

How do I make 1ug/mL of a stock solution from 10mg/mL of a mother solution?

I have a liquid compound. The volume is 100uL. On the vial of this solution is mentioned 1mg. On the data-sheet is mentioned 10mg/mL. I want to make 1ug/mL of stock solution in ethanol. How much...

21 September 2020 4,815 1 View

Can all network UE decode the information transmitted by a eNB through Physical Downlink Control Channel (PDCCH)?

A wireless network consisting of two UEs (A & B) and an eNB. The eNB transmits control information to UE A through PDCCH. Can UE B also decode this information? Or is it permissible for UE B...

07 August 2020 2,628 2 View

What is the Distance Distribution of random point in a clustered environment?

How to find the distance distribution of a random point in a cluster from the origin? I have uniformly distributed cluster heads following the Poisson point process and users are deployed around...

04 March 2020 6,134 3 View

Minimum ITS sequence length required to accurately identify the Genus in microalgal species?

The microalgal ITS region has about 600 base pairs in total. Are 400 bases enough to identify the genus of the organism? Can it be used to hint at the specie too? I am getting 97% query cover and...

18 January 2020 2,357 3 View

Is hardware support for Dsp C6000 available in MATLAB/SIMULINK ?

I'm currently working on a lab project of DSP and I need to connect Matlab with DSP 6713 kit. But hardware support for c6000 in Matlab's latest versions become obsolete now. and when I try it in...

16 December 2019 866 4 View

How do we differentiate between an organometallic compound and an MOF?

How can an XRD or an FTIR Pattern help to identify their nature if it has not been reported previously?

04 December 2019 3,831 3 View

Can someone recommend a platform to simulate MAS for microgrid applications?

Many researchers use JADE to simulate MAS. I am after a more graphical programming alternate. Don't want to spend time learning JAVA.

05 August 2019 367 3 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

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22 February 2021 9,932 11 View