Are there any tips for homogenizing a single mouse inguinal lymph node for total RNA isolation?

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Has anyone else received an email regarding payment for citation?

I recently received this email message: "Dear Colleague, I trust you are well! I am writing to propose a mutually beneficial collaboration. I have many research papers that need citations. If...

27 June 2024 9,627 1 View

Do you have a protocol for stimulation of mouse B cells by PWM?

Looking for a protocol for the stimulation of B cells with pokeween mitogen

22 June 2024 4,882 4 View

Which standards, frameworks or best practices for AI governance do you know?

Frameworks for AI governance The use of artificial intelligence in the company requires its control and monitoring by the company's governing bodies due to legal and regulatory requirements....

17 June 2024 8,956 5 View

Can someone explain the intuition behind, Blue stars are small, but wavelength is big. Red stars are Big but their wavelength is small?

can someone use quantum mechanics to explain the intuition behind this phenomenon?

07 June 2024 9,584 2 View

Can delirium cause brain damage that is similar to damage caused by a stroke?

Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...

07 June 2024 8,723 2 View

How does one get and add a DOI to an existing file upload?

to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?

06 June 2024 5,095 1 View

Which form/type of epigenetic modification is most closely associated with transgenerational effects?

Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...

31 May 2024 4,613 3 View

Help! Alizarin Red not staining?

I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...

28 May 2024 4,068 2 View

How can i get available datasets for Kaduna metropolis at least 2 busy 4-way intersections for research purpose?

I am working on vehicle pedestrian safety at 4-way intersections with traffic signal control lights. I need dataset available for Kaduna metropolis busy 4-way intersection with pedestrian count...

21 May 2024 4,872 1 View

What is the rate of acceptance for Business and Professional Communication Quarterly?

Where can I find Business and Professional Communication Quarterly's manuscript acceptance rate?

18 May 2024 1,303 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View