Are there any specfic immuno-markers for somatic motor neurons?

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Monica Mccullough

Thus far, Choline acetyltransferase (ChAT) is still the most common used marker for somatic MNs.

Wouter Everaerts

Unfortunately ChAT will not allow discrimination between somaticMN and parasympathetic fibers. Any other suggestions?

Ignacio Hernandez-Morato

Two ideas:

One. Use retrograde dyes injected into Prostate

Two. You can use ATF-3 in combination with ChAT after injury of the nerve that innervate the prostatic region. ATF-3 is over expressed after axotomy. I think ATF-3 is not expressed by parasympathetic neurons (check it). ChAT is not expressed by sensory neurons.

Mohamed Najimi

I agree ATF-3 in combination with ChAT

Jai Ghosh

there is no better marker than acetyl choline esterase. In fact there is an auto immune disease of acetyl choline esterase called Myasthenia gravis.

David I Hughes

Try ChAT, or CGRP as an alternative. You may also wish to try transganglionic/retrograde labelling by injecting CTb into the peripheral nerve, but this is an added level of complexity.

Gong Ju

ChAT is a marker for somatic motor neuron, if one can clearly define it as somatic motor neuron. The paraysmpathic and some interneurons are ChAT immono-possitive. A more espcific marker is HB9.