So far I am only aware of one modern karyotypic study on Nemerteans (Chen et al. 2007), and some reports on chromosomal numbers. But what about ploidy?
I'm dealing with a diverse genus (>50 spp) that contains a great deal of cosmopolitan fungal species, and I have a set of DNA sequences of one to three genes. I would like to know if there is a...
03 April 2014 2,845 10 View
Since the number of estimated species for the Fungal kingdom is constantly changing, the numbers that I've found in not-so-recent literature may not be adequate anymore. I was wondering what are...
02 March 2014 6,703 1 View
I'm very new to this subject, but my current task is to try to detect any level of recombination in a supposedly asexual haploid species (as a proxy for cryptic sex, maybe). I have information for...
02 March 2014 3,211 0 View
I was wondering if there are any methods available to infer the ploidy level of a non-model species using, say, transcriptome data or something similar. This would be ideal if you have no...
31 December 2013 5,289 16 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View