DEEPAK:

MAT: are you using it for humans or animals sera?, If animals Vaccinated domestic or un-vaccinated?, Ig G detection or IM G.. There are some Elisa Kits out there but they do not work a the Serovar level in the same way or range, much more useful in the human Scenario.

Second Mat it is not a Molecular Technique, and for that you requires isolates and Bacterial DNA an up to this day is a little different than the PCR Techniques used for other bacteria , due to Leptos complexities.

May I suggest , for you to look for this two paper to get a comprehensive review on Lepto and the available tecniques.

History of leptospirosis and leptospira.

Adler B.

-Curr Top Microbiol Immunol. 2015;387:1-9

Leptospira and leptospirosis. Adler B et al. Vet Microbiol. (2010)

Look at the ILS web and subscribe (free) ..International Leptospirosis Society Inc. (ILS), there most of the world experts on Lepto are in contact and you will find the support and guidence

http://www.med.monash.edu.au/microbiology/staff/adler/ils.html.

Kind regards

Thanks for your reply. I am working on leptospia zoonoses so i want differ the animal and human leptosprira pathogens at  species level without using MAT.  I will approach to ILS.