PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

What statistical test do I use when analysing data from the social physique anxiety scale?

I am researching the effect of social media on social physique anxiety of young adults. What statistical test do I use when analysing data from the social physique anxiety scale? (Leary, 2013)

03 February 2021 2,618 5 View

What are the negative aspects of the COVID-19 epidemic on the well-being of young people who are unemployed?

What has been your experience in your respective country?

22 December 2020 663 3 View

Is coronavirus affects children or not?

It has been seen that many deaths of older people happened due to coronavirus, younger was found infected and then recovered yet no any case or less cases or children affected by coronavirus was...

06 December 2020 5,012 18 View

What modeling software can be used to analyse fate of pesticides using a fugacity multimedia approach?

Hello, I am trying to understand the fate of pesticides based on the fugacity multimedia approach. Is there software for such modelling? I know SWAT can be used for watershed-level pesticide...

15 October 2020 1,424 3 View

Do you know if, in your country, some specific measures regarding young people have been put in place since the beginning of the pandemic?

Dear colleagues, I have been asked to write an editorial about young adults facing the pandemic and measures to be taken to mitigate social risks (in France). I am looking for some international...

14 October 2020 8,202 9 View

What are the underlying health conditions that increase vulnerability to coronaviruses and risks of higher mortality among young adults and children?

Underlying health conditions that play a role in increasing vulnerability to coronaviruses and risks of higher mortality among young adults and children

13 September 2020 7,107 7 View

Where to publish systematic review protocol?

I would like to prepare a systematic review protocol. I am wondering about any journals that will publish a protocol without charges from the authors? It does not have to be open access. The topic...

23 July 2020 4,537 8 View

Are the temperature screening alone at most of the entry and exit points sufficient during Covid19?

21 June 2020 3,267 21 View

Where to find recovery rate and death rate of COVID-19?

Some people, even doctors say it's not a serious disease, it's like seasonal flu, which could cause death. Where to find the recovery rate and death rate of COVID-19 in respect of older adults...

16 June 2020 6,546 12 View

Journal without a word count in the public health/general practice/older adults field?

I'm looking for a recommendation of a journal with a high word count (above 6,000 words) related to any of the following topics: public health, general practice, primary care, older adults,...

20 April 2020 1,093 2 View