Target organisms are Rickettsia and related bacteria. Also looking for collaborators on this for present and future related research projects. Interested people please write to [email protected].
Is there a software (non command based programs) to make a schematic diagram of gene (4500bp) and label multiple primer and probe binding sites and amplicon regions? An example diagram in the...
05 February 2017 6,213 4 View
I am using 12% gel. Tissue was lysated using RIPA buffer and doing semidry transfer.
19 October 2023 6,043 2 View
I performed nested PCR in tick sample for sequencing rickettsia. Second round of nested PCR use DNA template is first round elute 10X and result have so much band (attach picture). Do anyone have...
11 July 2023 1,633 2 View
The protein extracts are frozen at -80 degrees, and we have tried to quantify by the Lowry method both before freezing and just after thawing and before loading the gel. Even if we quantify the...
13 June 2023 7,661 1 View
I am trying to run a western blot on catechol-o-methyltransferase (COMT) in the rat brain. All of my other proteins of interest (TH, GAPDH, Vinculin) are showing up exactly where they're meant to...
13 November 2022 2,791 7 View
Good Evening, I have a question about the normalisation of my results when measuring phoshorylated proteins. Example: EGF stimulation experiment: I measure phoshorylation of AKT as a function...
30 December 2021 3,880 1 View
Can anyone recommend a suitable brand for ELISA positive control for rickettsia (e.g. Q fever, spotted fever, murine & scrub typhus)?
28 November 2021 6,649 1 View
I have a double signal from vinculin, one between 150-100 that corresponds to the information on the data sheet, but another very intense band between 75-100. Could someone tell me why?. It is...
06 October 2021 5,771 1 View
Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...
07 January 2021 6,923 3 View
I'm working on subcellular fractionation. I use Vinculin, Lamin B1, and COX IV antibody for cytoplasmic, nuclear, and mitochondrial fraction, respectively. However, I'm not quite sure if I have...
12 October 2019 6,197 3 View
I am working on detection of rickettsia in ticks, while I use a primer whose annealing temperature in the original paper 48, I always get non specific bands without appearance of the specific one...
04 May 2019 9,188 5 View