There are many ways to estimate chlorophyll content in plant leaves ,including SPAD unit method and CCI ,so that can any one provide me weather there are any converting factor connect between these two methods of measurement>
D. Bassim taking a patent in this studay.
I did not touch correlation between spad and Chlorophyll content
Depends on the plant's leaf thickness
Waxy layer
Hairs on the leaf surface
Although I am not familiar with the correlation between SPAD unit and CCI you mentioned here, here is an original paper by Dr. Markwell and his colleagues (Markwell et al. 1995) to get you started. This is an equation I have used whenever I need to do the conversion between the SPAD unit and total chlorophyll content on wheat, pearl millet, and corn. I believe someone else has asked a similar question(s) before, and I also shared my thoughts on the topic. You might search and see the answers he/she has received, which might be useful to you as well.
In addition, I also looked into the factors affecting the accurate conversion (or correlation) of the SPAD unit to Chlorophyll content several years ago. I believe that leaf thickness was one of the main concerns, which is not a major concern in general for our common agricultural crops (C3 and C4 plants). As we all know, the plants with thick leaves are the desert [or CAM (crassulacean acid metabolism photosynthesis)] plants, [perhaps some SAM (submerged aquatic macrophyte) plants as well]. If you are looking for the factors influencing the correlation, you could try a literature search on "leaf thickness and SPAD unit", and see whether the results are useful to you or not.
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