In ruminants, nutrient inputs are first to fermentative digestion by ruminal microorganisms. The microbial fermentation products eventually become available as energy (volatile fatty acids) and protein (microbial cells) for animal tissue metabolism. However, ruminal fermentation also produces methane (CH4), carbon dioxide (CO2) and ammonia (NH3). Methane production in the rumen is an energetically wasteful process, since the portion of the animal’s feed, which is converted to CH4, is eructated as gas.

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