Are people with recessive traits less likely to be carriers of the corresponding dominant genes(to those recessive traits)? How? Why?
Hi I am trying to model a cutting problem with a blade and comparing the results with and without ultrasonic vibration. From experimental results in our group, the use of ultrasound can...
13 June 2024 7,461 4 View
I'm conducting research on photoplethysmography (PPG) signals obtained from smartwatches worn on the wrist. The main goal is to analyze the PPG waveform and extract key fiducial points and...
13 June 2024 3,286 0 View
I am running some simulations in Abaqus to extract the stiffness matrix of a FE model with geometric nonlinearity. I want to provide to Abaqus a set of nodal displacements (all the dofs in the...
28 May 2024 4,797 2 View
Hello together! I have some time-series for tree-rings from dating from 1850-2016 and diameter at breast height (DBH) from 1996-2022 for the same plot, however the sampled trees for tree-rings...
16 May 2024 5,295 2 View
I think so because The Random Slate Theorem
03 May 2024 3,082 7 View
I am currently trying to get a Skyscan 1072 micro CT to work again. After the mainboard on the original PC died, we tried to set the machine up on a new computer. We managed to control the source,...
28 April 2024 381 0 View
Is it worth using a paired t-test on pre and post survey data with 12 or less responses? Or should one rely more on the mean and standard deviation of the two data sets to evaluate the survey...
27 April 2024 8,703 8 View
1)Religion(or at least spirituality) calms my mind, particularly, the built in belief of only one reality. God created the only reality. The afterlife is another dimension. We have free will but,...
25 April 2024 10,055 0 View
I have 217 preprocessed BrainVision matlab files (.mat) with ECG data in them. I've been having trouble creating a study for these. I know BioSig works for ECG data, but it doesn't seem to work...
21 April 2024 1,028 2 View
I have 25 folders for 25 participants, all with .mat files inside that are preprocessed. Most of them have the same amount of .mat files - a couple participants didn't finish the study, so they...
17 April 2024 1,897 3 View
Hello everyone, I'm currently working on calculating the relative levels of mtDNA using qPCR by comparing the Ct values of a mitochondrial gene (nd1) and a nuclear gene. According to my...
16 July 2024 5,508 0 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
26 June 2024 5,913 3 View
Hi Everyone, I'm reaching out for some guidance regarding conducting a comparative Ct analysis with the ABI StepOnePlus system, and I hope you can help shed some light on my dilemma. Here's the...
11 June 2024 4,044 3 View
I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...
10 June 2024 5,941 3 View
I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...
06 June 2024 941 1 View
I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...
04 June 2024 6,924 0 View
Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures...
03 June 2024 6,795 1 View
Hello - planning a SYBR Green qPCR study. I have primer efficiencies, including for reference genes, from 90-105% I am planning to use efficiency correction in my calculations - do I correct for...
15 May 2024 3,370 0 View
cq values for my reference coming like 22 and cq value of my target gene coming as 23 and 24 around? can i considered this value for future calculation? or what else i can do to get better cq...
14 May 2024 6,081 6 View