Recently, we started working on DENV2 in our lab. I had received a sample of the virus from a researcher with unspecified viral titre. I cultured the virus on Vero cell monolayer by adding 100uL of the virus sample that I received. Even by the end of 5-6 days of incubation there was no visible CPE. But I had to aliquot and stock the supernatant as per protocol. Plaque assay results with this new viral stock gave me really high viral titres (6.9 x 10^10 pfu/ml).
The researcher who had sent the virus said that he had sent the virus stock of 10^6 pfu/ml. Is such high viral titre possible? Is it because I started with a random volume of the original stock?
I agree with Rodrigo Guabiraba. To confirm the potency of the any new isolate, one has to compare with the known standard isolate to know whether your system works or not. Otherwise, we will have too many doubts to clear. good luck. lalitha kabilan
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