Predicting Depression Levels Using Social Media Posts

The use of Social Network Sites (SNS) is increasing nowadays especially by the younger generations. The availability of SNS allows users to express their interests, feelings and share daily...

01 January 1970 4,400 2 View

What is the role of asparagine in cancer?

Asparaginase is useful in the treatment of leukemia as it degrades asparagine. What role does asparagine play in the proliferation of cancer cells?

01 January 1970 4,720 3 View

Impact of the Covid-19 pandemic on the Aquaculture and Fisheries sector.

Hello all, With all the lockdown regulations, sales and trade may not be as it was before, goods and services are impacted; fish and fish products and services are not left out. Would you...

01 January 1970 3,700 13 View

The Role of Renin-Angiotensin-Aldosterone System in the Severity of COVID-19 in Diabetic/Hypertensive Patients

Hypertension, diabetes, and cardiovascular disease are the most prevalent comorbidities in patients with COVID-19. Although they do not appear to affect the infectivity of the virus, they do...

01 January 1970 8,859 8 View

The 'Biochemistry' of permed hair

Is there more to a permed hair than the alteration of the disulphide bonds between adjacent strands of protein in hair?

01 January 1970 8,589 3 View

'Biotechnology'- A textbook authored by V. Satyanarayana

Please who can help with copy of this textbook in PDF?

01 January 1970 5,980 5 View

Cancer cells and metastatic phenotype

Why do cancer cells use distinct transcriptomic and proteomic programs to reach the same metastatic phenotype?

01 January 1970 4,948 3 View

Biotechnology: Do the Benefits Outweigh the Downsides?

The major apprehension of genetic engineering is that through recombinant DNA experiments , unique micro-organisms or viruses (either inadvertently, or sometimes deliberately for the purpose of...

01 January 1970 965 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Multicloning in pRMC2?

Hi all, I want to express several genes in S.aureus by using the pRMC2 expression vector. I want to try to subclone some genes already cloned into pRMC2 (and their own tetO promoter) and put them...

01 February 2021 5,108 3 View

Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Why is there no fluorescence if GFP-expressing vector is present and sequence is correct?

We recently constructed a plasmid vector with a high-copy number ori, kanamycin resistance, and eGFP under control of the lac promoter but lacking the lac operator. However, the cells are not...

19 January 2021 7,834 2 View

Can anyone please explain about the renaturation of two different subunit of recombinant protein?

Thanks in advance to everyone who answers my question. I want to raise a recombinant N-methylhydantoinase protein by pET expression vector system. The protein is a tetramer of two identical small...

18 January 2021 1,026 2 View

Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Can somebody recommend a dual expression vector?

09 January 2021 2,679 2 View