are Amoxicillin and Ampicillin will be denatured when it autoclaved in agar nutrients?
You can get benefit from the following links :
1. https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/a0166pis.pdf
2. http://diyhpl.us/~nmz787/pdf/Journal_of_Antimicrobial_Chemotherapy_1995_Traub_149-154.pdf
You can get benefit from the following links :
1. https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/a0166pis.pdf
2. http://diyhpl.us/~nmz787/pdf/Journal_of_Antimicrobial_Chemotherapy_1995_Traub_149-154.pdf
Hello, Yoga,
The answer to your question is yes. Heat-sensitive antibiotics are destroyed by autoclaving. In preparing microbiological medium supplemented by antibiotics, you normally autoclave the medium first and then wait for it to cool down before adding your heat-sensitive antibiotic/s. Keep in mind that you should be using sterile and filtered antibiotic stock solutions.
Hope this helps,
Arizaldo
Antibiotics will usually get denatured during autoclaving. It is most appropriate to add them to a medium after the autoclaving step.
yes prof. addition of antibiotic should be after autoclave the culture media.@mohammad abdullah
Yes of coures all antibiotics denaturated when we autoclavig it. If you need to adde antibiotics to media you should firstly autoclaving media and when media rech 45C you adding antibiotics
Some antibiotics with melting points higher than the autoclaving temperature of 121C can be autoclaved. Examples of such abound eg gentamicin,chloramphenicol etc...... This is the concept adopted by some media manufacturers, example SDA plus Chloramphenicol, SDA plus gentamicin etc. No one separates the antibiotics components before autoclaving such media and they still work after autcoclaving.
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