Are 5-HT1A receptors located on hippocampal interneurons?

More Manivannan Subramaniyan's questions See All

How to check spread of AAV2 virus that doesn't express fluorophores?

Hi, I am injecting AAV2-Retro virus (AAV2-retro-EF1a-DIO-FLPo-WPRE-hGHpA from Addgene) that carries cre-dependent flp recombinase, in the mouse dorsal hippocampus and I need to verify that the...

08 November 2020 9,559 3 View

How to solve Inherent Conflict in AR - Challenge in Interaction?

Our recent research shows that AR systems have inherent conflict while interacting with virtual objects. We termed this new conflict as Virtual Kinesthetic Conflict (VKC). This conflict is very...

21 June 2020 4,876 1 View

What is the most important challenge in Biomechanics?

Biomechanics face grand challenges due to the intricacy of living things. We need multidiciplinary approach (mechanical, chemical, electrical, and thermal ) to unravel these intricacies. We need...

25 May 2020 3,374 20 View

May I know, which statistical analysis and conceptual framework will be suitable for my study?

Helo, I am interested in looking into Secondhand smoke exposure and support level towards smoke-free legislation. I am planning to use secondary data. Can anyone advice on which statistical...

23 May 2020 6,381 2 View

How to evaluate the In vitro study for coronavirus (COVID 19 Inhibitory activity)?

Dear Researchers .... Need help for COVID 19 drug discovery. Recently, COVID-19 main protease crystal structure was deposited in the protein database (PDB ID: 6LU7...

08 March 2020 3,853 4 View

How to upload homology modeled 3D protein in online free protein bank website?

Dear research professors and scholars, I have developed a novel 3D protein structure (mutant DNA Gyrase enzyme of antibiotic-resistant E. coli) by homology modeling technique. Because this type of...

21 September 2019 5,159 2 View

409bp is good for real time PCR?

I designed a primer and probe for real time PCR using (https://www.eurofinsgenomics.eu/en/ecom/tools/qpcr-assay-design/) this website with the bp size shows 143. after i received the primer i did...

19 July 2019 7,963 10 View

What are the values of SIG-SAS etc. to be given in material model of the shape memory alloy in ANSYS Mechanical APDL?

I am doing project related to Shape memory alloy and I want to simulate the shape memory effect in APDL. I don't know what to be given in the material model of the shape memory alloy given in APDL...

04 February 2019 9,505 4 View

Best method for tracking small objects in Virtual Reality?

What is the best algorithm or technique for tracking small objects in virtual environments. Best in the sense of tracking resolution, latency, and cost.

29 October 2018 9,137 3 View

What is the most important technical challenge in Virtual Reality?

There are many technical challenges in VR/AR. Among these, which is the most important technical challenge without which VR/AR will miss the mass market? Let us discuss.

16 January 2018 5,992 22 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View