Wild type seeds are germinating and growing fine in the same soil mixture.
Pramod Sargar
The mutant seeds may not germinate because of mutations in genes essential for seed germination, such as those involved in GA signaling. As the dose of mutagen/mutation increases, the disruption to these genes is more severe, resulting in a lower germination percentage.
You can try a few things:
- Stratify the seeds at 4°C for several days before planting. This can help to break dormancy and improve germination.
- Use fresh seeds for mutation treatment. Because Arabidopsis seeds have a relatively short shelf life.
- Use a sterile growing medium to avoid fungal contamination.
- Plant the seeds at the appropriate depth. Arabidopsis seeds should be planted shallowly, just below the surface of the growing medium.
- Water the seeds regularly, but avoid overwatering
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Variyata Agrawal
Thanku Mr. Promod.
I have kept the seeds sown on soil at 4° for two days before keeping it at plant growth chamber still they ain't germinating.
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Andrew Paul McKenzie Pegman
You need to determine if the seeds are viable first using the flotation test. Viable seeds will sink in water. Then use only those :)
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