Hi dear colleagues.
I want to apply bootstrap and improving the phylogenetic trees in my study. phylogenetic comparison was done by distance matrix/UPGMA analysis using Kimura 2- parameter by MEGA7 software package program. can any one help me to apply the bootstrap value to support the trees?
Hi Mallam
Can you explain more. actually I have not enough experience in phylogeny
If you want to have a statistical support for your branches, you must do an analysis such as maximum likelihood. You can do it and incorporate the bootstrap analysis in MEGA, unfortunately it must be set before running the analysis, so you will have to repeat it. Depending on the number of sequences you have, you can select 500 or 1000 pseudo-replicas.
Mr Bayes uses Bayesian statistics, it also gives you statistical support for the branches, but you should know what kind of information you want to extract from the tree.
MEGA does not have Mr Bayes incorporated, so you should install it (preferably on a Linux machine)
Best regards
Even (If you want it) you don't need to install the software in your computer. You can use the science gateway CIPRES, you can run a lot of statistical analysis and they have the manuals of each one. But, as the other colleagues said, you need to know what information you want to obtain from the phylogenetic analysis to know what software could help you
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