Abbas ali Husseini

4 Questions 2 Answers 0 Followers

Questions related from Abbas ali Husseini

Applying the Bootstrap in Phylogeny Reconstruction ?

Hi dear colleagues. I want to apply bootstrap and improving the phylogenetic trees in my study. phylogenetic comparison was done by distance matrix/UPGMA analysis using Kimura 2- parameter by...

03 March 2018 9,915 4 View

Troubleshooting of real time PCR

I want to develop a HCV viral load determination based on quantitative real time pcr. I have used light cycler 480 and MGb probes and Ez kit. I have used a dilution of 1e7 up to 1e1 as a standard....

04 April 2013 7,402 1 View

Any suggestion for PCR from samples with very low concentration of DNA?

Hi every body. I work with HBV patient samples with very low concentration of viral DNA. I wana amplify a fragment of HBV genome but samples DNA concentration is too low. what can I do freinds ??

01 January 1970 1,087 5 View

Optimized PCR dosent work?

I wana amplify a rigon of about 900 bp of HBV genome. my samples collected 2-3 years ago and saved at -80. PCR conditon has optimized with fress samples but unfurtunatly dose not work seccessfully...

01 January 1970 6,565 4 View