How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View

How do you create a 0.5M EDTA disodium salt solution at pH=7.4?

I have been trying to make a 0.5M EDTA solution by dissolving EDTA disodium salt dihydrate in 500mL of purified water and adjusting the pH to ~8.0 so that the salt dissolves. To aid in the...

12 October 2020 3,242 2 View

How can I increase the length of DNA fragments recovered from QIAamp DNA Micro Kit?

Hi everyone. I'm performing DNA extractions for pools of ~10 individual Daphnia magna (water fleas) using the Qiagen QIAamp DNA Micro Kit. I use a pestle to homogenise the Daphnia before entering...

07 September 2020 9,956 1 View

Where could I find3D resistivity/conductivity (ERT) opensource data?

Hi, I would like to know whether is there any open source 3D inversion resistivity/conductivity geophysical data (ERT) available. If yes, how to access it?

06 August 2020 8,209 3 View

Is it normal to get different results when you try to simulate a paper your are basing your research on?

I am simulating AODV and AOMDV in NS 2 to compare their performance in terms of Packet Delivery Ratio (PDR), Throughput, Delay and energy consumption, in order to propose a better algorithm. The...

18 July 2020 9,755 9 View

How can I assess a method that returns high-expressed genes?

Hello, I working with a method that finds high-expressed genes in a single-cell experiment. How can I assess such method? I know I can compare it with the ones in literature (t-test, regression),...

22 February 2020 3,023 2 View

How should I hydroxylate tungsten powder for organosilane grafting?

I am having trouble depositing MPTMS/APTES onto tungsten and I am concerned there are not enough hydroxyl groups on the powder surface. So far I have been exposing tungsten to water to hydroxylate...

04 February 2020 4,677 2 View

Is it possible to perform a meta-analysis for an index value?

Dear researcher community. I want to ask if it's possible to perform a meta-analysis in an index, I mean, in value without standard deviation. For instance, in the regression model, many indexes...

04 December 2019 3,797 1 View

PLS-DA or LDA for NIR analysis?

I was wondering, for discrimination or classification propose is better use LDA instead PLS-DA in NIR data?. What analysis will works better in this kinds of data?. And why? Thanks so much in...

15 October 2019 8,409 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View