You could use a piece of paraffin. Just need a sharp edge in order not to be so dirty and use it like the pap pen. Best of luck!

D

Hi!

Are they cells in suspension cultured? If they do, we do the staining with an intracellular flow cytometry protocol and finallywe  put the cells on the slide in aprox. 10 uL of permeabilization buffer. Then we spread the cell suspension, let the cells to dry and add the mounting medium and the cover glass. 

So the dried drop defines the cell area. 

I don´t know if this will be useful for you.

Good luck!

You could use a piece of paraffin. Just need a sharp edge in order not to be so dirty and use it like the pap pen. Best of luck!

D

PAP pen from sigma is better. I am using this. 

Hey Mari,

Now I'm using Super Pap Pen from Invitrogen and It works very well, althouhg I have to do again the hidrophobic line after PFA fixation. The worst thing is the cost, very expensive.

Best,

AnaV

I think long time ago the glass slide was just scratched around the area of interest. I saw one researcher constantly doing that. It did not work for me though.

I like Daniel’s advice. Just do not have pap pen now. I will try it out And let you guys know.