Kateryna Bilotkach

5 Questions 12 Answers 0 Followers

Questions related from Kateryna Bilotkach

High molecular weight tail in NGS library for ChIP-Seq?

Hi everybody, Could anybody please reflect on what I see on the tape-station report and how it might affect the sequencing? I have prepared libraries for ChIP-Seq (input, 3 IPs and IgG ctrl)....

03 March 2019 7,984 1 View

How to combine data-sets for AP-MS data?

Hi, Could anybody please help with the basic understanding how to process AP-MS (affinity purification mass-spectrometry, e.g. mass-spec done on the pull down with an antibody of interest) data. I...

02 February 2019 4,251 4 View

IP optimisation for Mass-Spec: can antibodies crosslinking to the beads prevent protein complexes capture?

I am preparing samples for affinity purification mass spectrometry (or simply IP followed by MS). The idea is to immunoprecipitate protein complexes, elute them and do LS-MS/MS on them. Here: A –...

11 November 2018 1,635 4 View

Do o need to isolate nuclei for X-ChIP?

Hello, I am improving my X-ChIP protocol (x stands for DNA crosslinked to proteins with formaldehyde). In my protocol I do not isolate nuclei. I just use the whole cell lysate prepared with a...

07 July 2018 3,239 3 View

How do I process proteins after co-IP?

Good time of the day, I need to perform co-IP (and X-ChIP with the same targets and antibodies) For co-IP i used Pierce IP/co-IP kit (#88805). E.g. that one that uses antibody crosslinked to...

06 June 2018 7,969 5 View