Hello, I just started a project trying to produce eucalyptus protoplast and we wish to regenerate plants from it. Can someone give any advice what is the best material to start protoplast production to have successful plant regeneration from it? Is there any protocol that you could share? Thank you!
Dear Camila
Please take a look at the following two articles. I hope will be useful to you.
- Isolation of protoplasts from different Eucalyptus species and preliminary studies on regeneration
January 1991Plant Cell Tissue and Organ Culture 25(2):133-140
DOI: 10.1007/BF00042184
C. TeullèresA. M. Boudet
Thank you! I already looked these publications and if possible I would like to hear more about other people's experience with eucalyptus or any other wood plant that is difficult to work with.
Biotechnological Approaches for the Improvement of Eucalyptus
October 2016
DOI: 10.1007/978-981-10-1917-3_11
In book: Plant Tissue Culture: Propagation, Conservation and Crop Improvement
Aggarwal DiwakarAggarwal DiwakarMondem Sudhakara ReddyMondem Sudhakara ReddyAnil KumarAnil Kumar
Dear Camila
Please refer to the following links and articles about the protoplast culture and plant regeneration in woody trees.
https://www.google.com/amp/s/www.researchgate.net/publication/307583000_Protoplast_isolation_and_genetically_true-to-type_plant_regeneration_from_leaf-_and_callus-derived_protoplasts_of_Albizia_julibrissin/amp
https://www.researchgate.net/publication/268047351_Protoplast_isolation_culture_and_application_to_genetic_improvement_of_woody_plants
https://www.google.com/amp/s/www.researchgate.net/publication/257040048_Plant_Regeneration_from_Protoplasts_of_Apple_Rootstocks_and_Scion_Varieties_Malus_X_domestica_Borkh/amp
You may also want to look at this article. " Inhibition of phenylpropanoid biosynthesis increases cell wall digestibility, protoplast isolation, and facilitates sustained cell division in American Elm" and other related articles from that lab... If you haven't already looked at them...
@Camilla, you may want to refer to articles of Dr. Praveen K Saxena's team on protoplast isolation and regeneration of American Elm, if you haven't already.
@Camilla, would also be interested in sharing notes on your experience in this area.... We have toyed with this aspect for a while....
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