Dear Suri

Please let us to know your field of work. Your request is too unspecific.

Regards

Respected Sir,

I have recently been registered for PhD in Environmental science. I chose Legionella as the organism to work with as here at my place there is no research done on this topic. Hence I want to know how to start with it here and the direction to go into, so as to write a novel thesis.

My supervisor's work is different, but, he encouraged me to chose this topic.I need proper guidance and advices from experienced professors like you to have a bright career ahead.

Thank you Sir.

I would recommend the book "Legionella protocols" , which include a comprehensive set of methods to use and apply with Legionella.

http://www.springer.com/us/book/9781627031608

Good luck!

Thank you Sir. Will definitely go through it.

Dear Suri

As apointed by Vincent maybe the intial approach is improving your analytical skills with environmental samples. Last year was aproved the last revision of ISO 11731, therfore is a good moment to initiate on that.

At the same time you can do prospective surveys in order to know the Legionella prevalence in your geographical area. Big buildings as hospitals and hotels, both are high risk systems.

Maybe you can put the focus on epidemiology or in mollecular detection methods as vPCR. It's a second stage that must to be in coherence with the scientific strategy of your institution.

regards

Ok Sir. Thank you. It is a very valuable information. I will keep this is mind and get back to you, once I am ready with a pathway.

Try to get some funding and register for the anual ESGLI Meeting in France this year. There you get a broad sprctrum of research and ideas.

http://esgli2018.univ-lyon1.fr/en

Cheers Markus

Legionella is a typical opportunistic pathogen in the drinking water distribution system. In order to work on Legionella, I think, first, you should know the basic skills such as qPCR, sequencing, bacterial stain, SEM, selective culture medium, et al. Then, you should decide that your research on Legionella is performed with pure cultural Legionella or envirionmental bacteria (such as drinking water). I suggest that you can start your work on Legionella by perfoming different disinfection processes on Legionella.

good luck.

Thank you Sir

Thank you Markus Petzold Sir for your advice....