I want to validate serine-282-phosphorylated Ab using MCF7, but without any luck so far. I am getting nonspecific bands but not the right size ( 67KD). I would appreciate any suggestions.
iam working on long memory time series data and i use lstm neural networ to train data and i want to understand how rmse is calculated is it calculated in each iteration separately or in each...
23 December 2020 6,740 4 View
Hello everyone, Is it possible to calculate the E/I ratio on single-cell levels only based on miniature signals (Excitatory and inhibitory)? If yes, then what parameters should I take to...
01 December 2020 9,715 3 View
Thanks in advance.
31 May 2020 732 6 View
Hello everyone, I am a PhD student with a deep learning background. I am trying to find more robust deep learning models and I just came through defining the problem as a minimax problem as the...
03 December 2019 9,260 12 View
Is there any examples of experimental type of questionnaire type ? I need scales to measure sustainable behavior, I'm thinking to use both experimental and questionnaire. Thanks in advance
20 November 2019 7,545 3 View
In case of falling rain in some day during solar drying experiment by Indirect solar dryer integrated with PCM storage unit, can I shut down the dryer and continue next day? then how can I...
26 August 2019 5,065 8 View
i need a simple method for treating wood with nanoparticles preferably without conditioning
12 August 2019 4,647 4 View
How to convert analog signal to digital data using python code?
26 May 2019 1,498 3 View
How to convert 2D images to 3D using python for medical images?
20 May 2019 722 2 View
There is a relationship between smoking and Parkinsonism , could MPTP be a degradation product of Nicotine.
20 August 2018 7,891 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
28 February 2021 2,534 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View