I want to validate serine-282-phosphorylated Ab using MCF7, but without any luck so far. I am getting nonspecific bands but not the right size ( 67KD). I would appreciate any suggestions.
I found this ornamental plant in the garden please help me identify this specie.
30 June 2024 4,499 8 View
karma
20 June 2024 6,189 1 View
Greetings, I invite you to participate in a research study aimed at exploring the impact of Artificial Intelligence (AI) on education, focusing specifically on its effects on staff roles, skill...
12 May 2024 424 2 View
I have two patterns one is pure Brucite and the second is brucite with my adsorbed material and I want to identify the impurities on the second pattern. Thank you for any help you may provide.
22 April 2024 7,592 0 View
26 February 2024 5,202 3 View
Abstract: This multi-faceted study delves into the realm of Artificial Intelligence (AI) in modern organizations, with a specific focus on its evolution, applications, and the intricate effects it...
23 January 2024 7,771 3 View
Can Chow test be used to test difference in mediation strength between two groups? If not, what test should be used for this purpose?
29 October 2023 4,199 0 View
I have a sample composed of two subsamples (say public and private companies) and a certain relationship is insignificant in both subsamples. If this relationship is significant in the collective...
22 October 2023 9,972 2 View
I know that we should use one tailed significance if we expect the direction of the effect of the hypothesis, but what about mediation? when we should use one tailed test if we are testing...
15 October 2023 9,896 0 View
I have a sample composed of two categories, say males and females, when I test a certain path it gives insignificant results in both categories, surprisingly, when I test the same path for the...
14 October 2023 7,616 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View