Please have a look at the following links.:

http://aem.asm.org/content/64/5/1700.full.pdf+html

http://plankton.jp/PBR/issue/vol03_3/0303_180.pdf

http://dx.doi.org/10.1016/j.mex.2015.04.006

Best regards  Vit

Thanks for the response but the papers you suggested have enumeration methods only, I am looking for methods I can use to culture and identify SRB and SOB. I need to be able to isolate the bacteria and use them in bioremediation trials.

Sorry Ngonidzashe Mangoma:

there are the links for identification of SRB and SOB

http://www.sciencedirect.com/science/article/pii/0043135468900547/pdf?md5=e431b798bbcd434834c7f4c9bab5b585&pid=1-s2.0-0043135468900547-main.pdf

http://www.microbiologyresearch.org/docserver/fulltext/micro/131/3/mic-131-3-631.pdf?expires=1487750119&id=id&accname=guest&checksum=1A3B7EFC069CFB5B8EED17506A8E661A

http://aip.scitation.org/doi/pdf/10.1063/1.4975944

http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/652/1/17_0028.pdf

http://www.jesc.ac.cn/jesc_en/ch/reader/create_pdf.aspx?file_no=2013250716&flag=1

 Even if we employ sulfate reducing bacteria for remediation of contaminated groundwater by heavy metals in commercial scale, we do not make cultivation of them, so we do not have a protocol for cultivation. 

Best regards

Vit