I am a beginner user of Neurolucida and Neuroexploler
I am placing different markers on a dendritic tree either on the dendritic shaft or onto the bifurcation points of that previously traced dendrite. Later after saving and trying to open the file by Neurolucida explorer to get the branched dendogram I found some problems:
1- some markers are shift away from its proposed position on the dendogram, for example away from the bifurcation point or even the shaft itself on which I surely attached that marker in neurolucida.
2-sometimes, some or all my markers can not be displayed on the dendogram even I checked marker counts /object markers on segment in the display menu of the explorer.
3- frequent difficulty to attach markers onto certain points of the dendritic shaft, for instance attaching markers every 50 microns using neurolucida but the dendogram displayed later with the markers has not accurate distances.
Which Version of NeuroLucida are you using?
Are you reconstructing from z-stacks or from live-images?
Maybe something went wrong during the sampling (e.g. sometimes it makes problems by uing oil-objectives or it may be possible that the camera ist not exactly adjusted to the x-y axis...)
Thanks you very much Dr.Oliver,
I had a problem of matching the placed markers into their points on the dendritic tree, the dendogram obtained is not accurate. later I found that the Z position of that markers was not matching with these points but now I fixed it.
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