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I am a beginner user of Neurolucida and Neuroexploler I am placing different markers on a dendritic tree either on the dendritic shaft or onto the bifurcation points of that previously traced...
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I usually do it by the immersion of each grid into UA for 30 mins-wash in bid-h2o 3 times in 9-well rack then again immersed in LN for 10 mins then wash as before, it was working well but...
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