What are the best activation markers for T cells in human lungs?

Our lab is looking to buy antibodies that are markers for activated T cells in human lung tissue. Has anyone tried something like this before? What did you use?

03 February 2020 339 2 View

How long do alexa dyes continue to fluoresce after loading neurons for long-term imaging?

Can anyone share their experience on the lifetime of different tracers or dyes? I am retrogradely labeling neurons with cholera toxin (CtB) 594 for chronic in-vivo imaging. I assume the lifetime...

28 October 2019 9,546 3 View

Can you reuse PDMS for cell culture?

We have cultured cells on PDMS functionalized with a Matrigel/Collagen mixture, and are wondering if new cells can be directly reseeded after enzymatically removing the original cells. We aren't...

28 November 2018 2,124 3 View

What is the typical distance from the surface of the skull to the brain in adult mice?

I am performing stereotaxic injection in mice. In the Paxinos Mouse Brain Atlas it appears that the depth is relative to the brain surface at bregma (z=0). The hole I drill in the skull is tiny,...

05 November 2018 2,738 4 View

Could anyone send me a FileMaker template to create a biological collection (bacterial strains, mutants, etc)?

I am planning to create biological databases for yeast, bacterial, strains and plasmids. I want to use FileMaker pro but I am a new user/ A template would be of great help to see how I can modify...

20 October 2018 6,708 1 View

What are 5 interesting topics that I should talk about in a paper about the advancement of artificial intelligence?

I am writing a paper in my CIS class on the advancement of artificial intelligence, and have come across a problem trying to think of some interesting topics. I am turning to you brilliant people...

17 September 2018 888 5 View

Does anyone know of dorsal horn proteins that are postsynaptic exclusively to C-fiber terminals?

25 April 2016 9,137 1 View

How can location be used to tailor news to individual consumers in a way that goes beyond such "lackluster" units of relevance as zip code?

22 September 2015 8,867 3 View

Does anyone have blue crab tissue or dna aliquots that are known positive for H. perezi or A. michaelis?

I am looking for positive controls of Hematodinium perezi and Ameson michaelis for use in molecular screenings of hemolymph for blue crab pathogens. Any help would be greatly appreciated.

09 December 2014 6,844 1 View

Does anyone know of good retinal ganglion cell specific markers/antibodies that also label dendrites?

21 October 2014 6,418 9 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

No title

01 March 2021 3,905 5 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View