Anyone have experience working on circulating tumor cells?

More AK Samadi's questions See All

How can I select in f2 segregated populations by molecular marker (MAS) in canola (Brassica napus)?

How can I select desired traits in f2 segregated populations by molecular marker (MAS) in canola (Brassica napus)? (for example markers associated with spring or winter type cultivar, yield-...

14 June 2024 9,525 0 View

What are the important use cases for a Cyberknife system?

Dear Friends I am planning to write a white article about the Cyberknife system and need your help. Since I want to write an effective article about the Cyberknife system, I have prepared some...

10 January 2024 515 0 View

Mxene Nanosheet synthesis ?

Hi deaer I have complatede many sucessful mxene etching. Synthes method is delaminate 1g Ti3AlC2 MAX phase in 18.6 ml %37 HCI fume, 1.4 ml DI water and 1 g LiF. After sysntheisi I washed and...

16 October 2023 1,105 0 View

Does anyone have more information on the NACA 2430 wind tunnel/CFD simulation?

It would make my essay easier to find information about this airfoil. Thank you anyway! #naca #aviation #engineering #airfoils #pilots #2430 #information #wind turbine #CFD

03 March 2023 4,566 4 View

Sir, I would like to publish an article regarding observational data(A cross sectional study among Ayurvedic physicians) in a wos indexed journal?

Kindly help me to suggest the journal names Which are wos indexed and fast publication with quality

17 February 2023 4,428 1 View

Do my numbers for area under stress strain curve make sense?

Hi, I'm conducting compressive mechanical tests on jello material on a rheometer HR20 by TA Instruments. The cross hatched top plate is 8mm in diameter and circular in shape. I'm testing hockey...

15 January 2023 2,162 3 View

Lucid dreaming cooperation request ?

We are working on a project about lucid dreaming. So far, the data were collected and we are going to start writing the manuscript. We are willing to collaborate with someone interested in our...

16 September 2022 8,863 3 View

In HFSS, How to relocate the radiation pattern from global coordinate to required coordinate position after simulation?

Dear Sir, We are using HFSS Simulator and trying to plot radiation pattern for two case, of a slot cut on waveguide wall as fallowed Case one: A slot cut on broad wall of waveguide located quarter...

10 March 2022 2,867 3 View

Request Data for Research?

Dear Researchers, My name is Hanan Samadi from the University of Tehran with a M.Sc. degree in Engineering Geology (Tunnel construction and machine learning). The general development of Artificial...

04 March 2022 1,063 5 View

What exactly is being modeled by the Negative Binomial Distribution for Differential Expression Analysis?

I understand how the negative binomial distribution is derived from the poisson and binomial distributions to "model gene counts for differential expression analysis." However, I don't understand...

12 January 2022 6,983 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View