I am searching for someone who did this kind of activity because i have some points I would like to clarify.
N.B: I am not asking for articles to be shared wtih me , I am asking for those who had done thos experiments by them self
Great question, thanks for asking.
Good evening everyone can anyone send me SMC files of earthquakes of different intensities for a simulation on Plaxis 2D. Thanks
02 March 2024 4,362 1 View
أخاطب السيدة المذكورة باسم Sarah Ayman بحذف اسمها من على الأبحاث الخاصة بى لأن هذا يعد انتحال علمى وسوف نبلغ جامعة حلوان بذلك
25 February 2024 7,614 1 View
We ask you to kindly change the information of the first name written incorrectly as Sarah Ayman to the correct name as listed in the article, Ayman S Taha. Sincere thanks and appreciation.
25 February 2024 8,634 1 View
I know that many reagents are used as activators or enhancers (such as ammonium salts and so on) in copper oxide minerals processing to facilitate their flotability during the flotation process....
28 December 2023 2,694 0 View
Usually neuroblastoma have low transfection efficacy (5-10%) when transfected with Lipofectamine 3000. So, this time I have tried electroporation with this condition, poring pulse: 175V, 2.5ms,...
23 August 2023 1,962 2 View
How to calculate the optimum annealing temperature for PCR?
15 August 2023 2,205 5 View
Any solid reference to why sometimes, especially at very low current densities, it takes too long to reach the charging voltage cutoff, while discharge takes place much faster?
03 July 2023 5,583 3 View
I need to provide a dataset for an upcoming article and they prefer a DOI number to be provided. Can l do this in ResearchGate given that the lab function has been removed?
20 April 2023 3,588 4 View
give me 3 problems
11 April 2023 6,197 5 View
Green chemistry
11 April 2023 8,194 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....
05 July 2024 5,860 1 View