How can I remove dead cells from viable ones in Expi293 cell culture?

Hi everyone ! I am working with Thermo Expi293 cells for a while for high-yield protein production in lab scale. Sometimes, viability of the cells decreases day by day although nothing was...

12 November 2020 1,102 5 View

Why is BAMM producing strange results with hundreds of identified rate shifts?

Hi, I am running BAMM on different Newick-format chronograms. While all other trees run perfectly fine, I am getting some strange results when using one particular tree. This tree originally had...

09 January 2019 6,779 2 View

Would you have a protocol to isolate non UV compounds from plants?

by using columns as separation methode

17 October 2017 5,427 3 View

Hi everyone! Has anyone any experience transfecting THP-1 cells with DNA plasmids?

I would like to transfect THP derived macrophages with a HIV-1 encoding plasmid. I wonder if any of you has any advice. Thanks for your help! Gabriela

31 January 2016 2,123 7 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View

ECG and children with autism ?

Does anyone have experience with collecting ECG data on children with autism? How was your experience? I'm interested in the tolerance levels for kids to have wires attached to their chests. What...

02 February 2021 5,850 3 View

What new can be added in the field of historical studies to implement more realistic programs?

What new can be added in the field of historical studies to implement more realistic programs in dealing with the events of history and interpreting them with scientific sincerity and competence?

26 January 2021 559 1 View

List any intelligent frequently ask questions among poeple regarding special need education and people with special need?

i need any question that is related to the special need education or the people special need (exceptional children)

25 January 2021 7,851 6 View