Anybody can say me how to make to prevent the development of fungi in an insect artificial diet ??

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What is the most suitable medium of ascomycetes fungi ??

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Pavel Dobeš

Hi, you could use for instance nipagin (methylparaben). It is commonly added to artificial diet of Drosophila. The proper concentration of fungicides is dependent on insect species/artificial diet that you use.

Charles Vincent

1) Add a fungicide or a fungistatic compound to the diet. 2) keep diet in a fridge until experimental use. 3) consistently use relatively fresh diet (say not more thank 3 months). 4) surface sterilize the fridge where diet is kept before storing the diet for a few months.