I am trying to section paraffin embedded human ligament at 20µm, but the tissue keeps breaking in the middle, with vertical lines throughout the tissue. Does anyone have any tips on how to resolve this issue?
I've tried a new blade, decalcifying in EDTA, a surface softener, and different temperatures.
Eric E Peterson
In my experience, paraffin does not do well at that thickness for the reason you state. I would see if the goals of the biopsy could be met with 10 or 5 micron sections instead.
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