I agree with the comment above, your kinesin may be poor quality or slow movers. What is the time lapse between images that you are taking? What concentrations of ATP have you tried? How old are the kinesin you're using?

Hi Arif and Yoli,

@Arif - Thanks for the suggestion and sorry for the late response. I am using epifluorescence microscope for visualizing microtubule motility.

I have not yet tried with a fresh batch of kinesin perhaps my kinesin could be bad. Also, in my opinion scavengers should not be affecting motility, though I am using the glucose oxidase/catalase/BME/glucose system for scavenging oxygen.

@Yoli - ATP conc. in the final solution is 1mM. The time interval was more than 180 seconds.

Thanks for the help.