Sample preparation automatic instruments.

I would like to recommend quantitative HPLC-photometic method in biochemical and analytical chemical laboratory.

If you handle with proteins, Microsequencer, which is also quantitative, should be employed (please see file; HepG2 fucoidan).

What do you want to control/measure?

use calibrated instrument and use internal standard

Hi. First, you need to calibrate the equipment with the standards and then make the analytical curve to measure the limits of quantification and detection.

Greetings.

If you are using chromatographic techniques you have to ensure the purity of your solvents to avoid false detections

In response to your question, I've uploaded to my profile two informal items about preliminary work that didn't get followed up.

In 10.13140/RG.2.2.17720.85763 I propose that HPLC column switching be brought up to the standard established for GC more than 50 years ago by D. R. Deans. Briefly, switching should be pressure-balanced (no pressure or flow transients) and have no valves in the flow stream. Commercial offerings don't yet seem to be entirely satisfactory because (as far as I'm aware) they still depend entirely on flow control and klunky valves. I think it should be possible to do better with modern measurement and feedback control techniques.

Still on the subject of chromatography, I propose that old work on adapting GC detectors, particularly the FID and its sensitised (thermoionic) derivatives, for use with small-bore LC and electrophoresis. There does not yet exist an adequately sensitive universal liquid phase detector for organic compounds. Link: 10.13140/RG.2.2.35336.93446

What do you want to measure and control in this analytical chemistry project?

There is always some deviation in analysis of gas sampling and analysis. when checked with wet analysis and with gas sensors. Also problem with diffrent test methods, especilly at mico level in petrochemical industry. Lot of products claims of accuracy levels do not when checked with two diffrent instruments and cross calibrated with manual methods. You can takeup one specific area in the idustry which is critical for the process and work out refinement/ accuracy to improve the product.