Any suggestion about a cfos antibody (marking cells with a good signal/noise ratio) to use with teleost fish fixed free floating sections?

06 July 2018 0 425 Report

I have used SC Santa Cruz Biotechnology monoclonal (E-8) c-Fos antibody (Santa Cruz, sc-166940) in 4% PFA perfused goldfish free floating sections. We have terrible results, both with DAB and fluorescence. We have tried also fast fos protocol.

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